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KMID : 0364219920350040456
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Korean Journal of Zoology
1992 Volume.35 No. 4 p.456 ~ p.465
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Association of the 94 KDa Glucose-regulated Protein with Immunoglobulin Heavv Chain Binding Protein (BiP)
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Kang Ho-Sung
Kim Han-Do
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Abstract
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The 94 KDa glucose-resulsted Protein (SH 94), one of stress Proteins, is a Ca2+-binding protein in the endoplasmic reticulum (ER). In this study, the possible effect of Ca2+ on the native conformation of grp 94 was examined. When the purified grp 94 was analyzed by Sel filtration in the presence of either EGTA or CaCl2, it was eluted with apparent molecular weight (MW) of 100 KDa in both cases. When similarly analyzed with microtome or cell Ivsate, however, srp 94 was eluted with apparent IW of 200 KDa in the presence of E6TA, while with apparent MW of 100 KDa in the presence of CaCl2, indicating possible association of grp 94 with one or more other proteins in the absence of CaCl2. Consequently, immunoprecipitation with anti-grp 94 was carried out to determine which proteins specifically interact with grp 94. It is sho%un that srp 94 may interact, in a Ca2+_dependent manner. with other proteins including BiP (grp 78) which is also a stress protein in the ER.
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KEYWORD
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94 Kda glucose-regulated protein, Ca2+-binding, protein, Bip (grp 78)
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